David Shaw*, Ali AlBarakat, Daniel Tran, Joni Tsukuda, Mandy Yim
Early Stage Cell Culture, Pharma Technical Development, Genentech, Inc.
Keywords: cell line development automation, clonally-derived, single-cell printer
In order to increase efficiency, consistency and flexibility, we embarked on completely automating the clinical and commercial cell line development (CLD) process using custom automated solutions. Automated single-cell cloning (SCC) and hit-picking workflows have been in use for several years and automate the most tedious steps in CLD. The SCC workflow uses plate imaging to document that our cell lines are clonally-derived. We added single-cell deposition to our plate imaging workflow to gain significant efficiencies over limiting dilution SCC. We use existing hit-picking automation to pick and scale-up single-cell clones from 384-well plates to 96-well plates, 24-well plates, 6-well plates and 24-well deep well blocks. We developed custom seed train automation to passage seed train cultures and set-up and sample fed-batch cultures in an unattended operation. Using the same seed train automation and custom consumables we are able to scale up to 300mL cultures in an automated operation. The implementation of the entire automated CLD workflow and our projected capacity of 50 – 200 cell lines per year with a very limited staff will be discussed.