Controlling variation in input materials for upstream Bio-processes is challenging. Often the individual components that are included in the media come from a variety of sources, which introduces variability. The mixing together of over fifty powder ingredients is a significant challenge in all industries, as uniform distribution of all components, particularly the minor components is very challenging.
The conventional testing required to assess whether a vendor is supplying a consistent product, from lot to lot is complex and labour intensive and thus restricts how much resource can be directed at measuring and understanding variation between lot to lot of the important materials.
A new technology, based on Raman imaging has been developed by H2Optix, that enables rapid screening of materials for consistency of composition. This technique is particularly valuable for complex powder blends.
Samples of the blend are dispersed on a sapphire window, and over an hour the Raman camera collects spatially separated spectra of the powder mixture, at a resolution of between 5 and 10microns. This enables identification of the particles in the mixture based on chemical signature, and “mapping” of the size of each particle. In this way the components of the mixtures can be identified, their mass estimated, and a cumulative relative composition calculated. The equipment developed by H2Optix can perform this analysis for multiple samples in an automated fashion, and requires no other laboratory equipment, and little operator intervention.
This presentation will provide a description of this innovative approach to tracking variation in culture media composition.