Cell Culture & Upstream Processing
Evaluating the Effect of Poloxamers on DNA Transfection Methods
Poloxamer 188, a common surfactant utilized in biopharmaceutical manufacturing, has undergone intense scrutiny in recent years due to lot variability impacts throughout the industry. Investigations into the cause of variability have resulted in an improved understanding of poloxamers, including surfactant-cell interactions within cell culture. The generation of highly productive mammalian cell lines for biopharmaceuticals is essential to enabling a high-yield manufacturing process, and there are several steps within the cell line development process that can utilize medium containing poloxamers. DNA transfection via lipofection or electroporation methods involves delivery of DNA across the cell membrane and integration of that DNA into a host cell genome to express recombinant proteins. Cell culture media or formulated buffers have been optimized to enable effic ient gen e transfer; however, the effect of poloxamers in these solutions has not been extensively studied. We have investigated the impacts of poloxamers on various transfection methods and subsequent culture recovery, and have developed optimized processes for generating highly productive cell lines.