Cell Culture & Upstream Processing
High Throughput Cell Line and Scale-down Upstream Process Screening
We present use cases demonstrating the automation of the development and assessment of mammalian cell lines and related upstream processes in high throughput, including seeding, selection, passaging, cryo-conservation, as well as batch and fed-batch processing and data analysis. We show how the full history of all clones can be tracked - from initial transfection all the way to their evaluation in bioreactor runs - and how this information can be combined with product quality and analytics data. These automation approaches include the integration with instruments, such as pipetting robots, colony pickers, bioreactors and bioanalyzers and deal with cell line and process development for both antibodies (IgGs, novel formats) as well as therapeutic proteins (e.g., fusion proteins).