Itai Benhar, Ph.D. - Professor, Molecular Microbiology and Biotechnology, Tel-Aviv University
We present a solution for correct pairing of heavy and light chains of bispecific IgGs; an engineered disulfide bond between the antibodies’ variable domains that asymmetrically replaces the natural disulfide bond between CH1 and CL. Bispecific IgGs where the artificial disulfide bond is placed in the CH1-CL interface are also discussed. A novel approach for precise evaluation of correct chain pairing by LC-MC-MS combined with chemical crosslinking is presented. Examples will be provided for some of these bsAbs and future directions of the study will be discussed.