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Senior Lecturer, Biochemistry at University of Cambridge
I am a senior lecturer in the Department of Biochemistry at Cambridge University. I completed my PhD in the department of Clinical Biochemistry, Cambridge, where I generated some of the first monoclonal antibodies to human creatine kinase isoenzymes and used them to develop two-site ELISAs for these important clinical markers.
I completed my postdoctoral training in the department of cell biology at Stanford, working with Perter Parham on the sequence of clathrin light-chains. At Cambridge, I continued this work in cell-biology, but extended it to study voltage-gated sodium channels and their regulation by auxiliary subunits and the application of novel proteomic methods to study membrane proteins in vivo. In all these projects, I have made extensive use of quantitative antibody-based assays, including ELISAs, developing and extending the principles exploited during my PhD. In particular, the use of enhanced amplification methods for signal detection.