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Michael Kierny received his undergraduate degree in Biology from Iowa State University and his PhD in Biological Sciences in Dr. Brian Kay’s laboratory at the University of Illinois at Chicago in 2014. His thesis work focused on using phage-display derived single-chain variable fragments (scFvs) to detect biomarkers of laser-induced retinal injury. He then spent 6 months as an intern at Roche in Germany working on ADCs before joining Dr. Saravanan Rajan’s group in the Department of Antibody Discovery and Protein Engineering at MedImmune in 2015. His current efforts are centered on developing single B-cell technology to generate natively-paired libraries for mining of the human antibody repertoire.
Library-scale Reformatting of Enriched Phage-display Pools to IgG for High-throughput Functional Screening
Phage-display selections using combinatorial libraries yield single chain fragment variable (scFv) binders that require individual conversion to IgG for functional assessment. In a method termed Screening in Product Format (SiPF), this process is streamlined by reformatting the selected library in emulsions to maintain the original VH-VL pairing and enable functional screening directly in IgG format. Examples will compare the performance and efficiency of traditional methods to SiPF.