How Big Are Antibody Libraries Really? How Do We Improve Them? Are We Accessing the Full Diversity?
In vitro antibody libraries have been used to generate antibodies against many different therapeutic lead targets. Theoretical and experimental analyses indicate that one would expect to select 1-3 antibodies from a 1e7 library. However, this does not appear to scale to larger libraries with diversities estimated to be >1 billion, suggesting either that libraries are less diverse than thought, or selection methods do not tap the full diversity. This talk will discuss the use of NGS to explore these issues and the application of NGS to the creation of improved antibody libraries.